Original Contribution IN VITRO HYOSCYAMINE AND SCOPOLAMINE PRODUCTION OF BLACK HENBANE (HYOSCYAMUS NIGER) FROM SHOOT TIP CULTURE UNDER VARIOUS PLANT GROWTH REGULATORS AND CULTURE MEDIA
نویسندگان
چکیده
Tropane alkaloids, especially hyoscyamine (HYO) and scopolamine (SCO), are widely used in medicine for their antispasmodic, anticholinergic, and sedative properties. This study was undertaken to investigate the root and shoot tropane alkaloids production from in vitro shoot tip (as explant) culture of Hyoscyamus niger under different plant growth regulators including auxins (IAA, 2, 4-D, IBA, NAA), cytokines (KIN ، BAP) with different concentrations (0.5, 1 and 2 mg.l -1 ) and two media of full and modified MS (1⁄2 NH4NO3 and 1⁄2 KNO3 compared to those concentration in full MS medium) treatments. Alkaloids extracted were analyzed by gas chromatography /mass spectra (GC-MS) analysis. The results showed that the highest alkaloid content values in root (HYO: 0.2 %DW; SCO: 0.076 %DW) obtained in full MS medium containing 0.5NAA and 1BAP. Whereas, in the shoot organ the highest HYO (0.394 %DW) and SCO (0.234 %DW) found on modified MS medium supplemented with 1BAP and 0.5IBA. It was obvious that HYO was the prevalent alkaloid in root and shoot tissues. The maximum total alkaloids (HYO + SCO) yield (0.269 mg.plant -1 ) was also obtained on full MS medium supplemented with
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